All Ca2+-binding loops of light-sensitive ctenophore photoprotein berovin bind magnesium ions: The spatial structure of Mg2+-loaded apo-berovin

doi:10.1016/j.jphotobiol.2015.11.012

KMS > iHuman研究所

	All Ca2+-binding loops of light-sensitive ctenophore photoprotein berovin bind magnesium ions: The spatial structure of Mg2+-loaded apo-berovin
	Burakova, Ludmila P.1,2,3; Natashin, Pavel V.1,2,3; Malikova, Natalia P.2; Niu, Fengfeng 3; Pu, Mengchen 3; Vysotski, Eugene S.2; Liu, Zhi-Jie1,3,4
	2016-01
发表期刊	JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY (IF:3.9[JCR-2023],5.3[5-Year])
ISSN	1011-1344
卷号	154 页码:57-66
发表状态	已发表
DOI	10.1016/j.jphotobiol.2015.11.012
摘要	Light-sensitive photoprotein berovin accounts for a bright bioluminescence of ctenophore Beroe abyssicola. Berovin is functionally identical to the well-studied Ca2+-regulated photoproteins of jellyfish, however in contrast to those it is extremely sensitive to the visible light. Berovin contains three EF-hand Ca2+-binding sites and consequently belongs to a large family of the EF-hand Ca2+-binding proteins. Here we report the spatial structure of apo-berovin with bound Mg2+ determined at 1.75 A. The magnesium ion is found in each functional EF-hand loop of a photoprotein and coordinated by oxygen atoms donated by the side-chain groups of aspartate, carbonyl groups of the peptide backbone, or hydroxyl group of serine with characteristic oxygen-Mg2+ distances. As oxygen supplied by the side-chain of the twelfth residue of all Ca2+-binding loops participates in the magnesium ion coordination, it was suggested that Ca2+-binding loops of berovin belong to the mixed Ca2+/Mg2+ rather than Ca2+-specific type. In addition, we report an effect of physiological concentration of Mg2+ on bioluminescence of berovin (sensitivity to Ca2+, rapid-mixed kinetics, light-sensitivity, thermostability, and apo-berovin conversion into active protein). The different impact of physiological concentration of Mg2+ on berovin bioluminescence as compared to hydromedusan photoproteins was attributed to different affinities of the Ca2+-binding sites of these photoproteins to Mg2+. (C) 2015 Elsevier B.V. All rights reserved.
关键词	Bioluminescence Coelenterazine Aequorin Obelin Calcium
收录类别	SCI
语种	英语
资助项目	[01201351504]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000368954300008
出版者	ELSEVIER SCIENCE SA
WOS关键词	CALCIUM-ACTIVATED PHOTOPROTEINS ; CRYSTAL-STRUCTURE ; SEQUENCE-ANALYSIS ; CA2+-REGULATED PHOTOPROTEINS ; RECOMBINANT OBELIN ; MNEMIOPSIS-LEIDYI ; AEQUORIN ; BIOLUMINESCENCE ; EXPRESSION ; CLONING
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1064
专题	iHuman研究所 iHuman研究所_PI研究组_刘志杰组
通讯作者	Vysotski, Eugene S.; Liu, Zhi-Jie
作者单位	1.Kunming Med Univ, Inst Mol & Clin Med, Kunming 650500, Peoples R China 2.Russian Acad Sci, Siberian Branch, Inst Biophys, Photobiol Lab, Akademgorodok 50,Bldg 50, Krasnoyarsk 660036, Russia 3.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, 15 Datun Rd, Beijing 100101, Peoples R China 4.ShanghaiTech Univ, iHuman Inst, 99 Haike Rd, Shanghai 201210, Peoples R China
通讯作者单位	iHuman研究所
推荐引用方式 GB/T 7714	Burakova, Ludmila P.,Natashin, Pavel V.,Malikova, Natalia P.,et al. All Ca2+-binding loops of light-sensitive ctenophore photoprotein berovin bind magnesium ions: The spatial structure of Mg2+-loaded apo-berovin[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,2016,154:57-66.
APA	Burakova, Ludmila P..,Natashin, Pavel V..,Malikova, Natalia P..,Niu, Fengfeng.,Pu, Mengchen.,...&Liu, Zhi-Jie.(2016).All Ca2+-binding loops of light-sensitive ctenophore photoprotein berovin bind magnesium ions: The spatial structure of Mg2+-loaded apo-berovin.JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,154,57-66.
MLA	Burakova, Ludmila P.,et al."All Ca2+-binding loops of light-sensitive ctenophore photoprotein berovin bind magnesium ions: The spatial structure of Mg2+-loaded apo-berovin".JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 154(2016):57-66.