In vitro expression and analysis of the 826 human G protein-coupled receptors
Lv, Xuechen1; Liu, Junlin1; Shi, Qiaoyun1; Tan, Qiwen1; Wu, Dong1; Skinner, John J.1; Walker, Angela L.2; Zhao, Lixia1; Gu, Xiangxiang1; Chen, Na1; Xue, Lu1; Si, Pei1; Zhang, Lu1; Wang, Zeshi1; Katritch, Vsevolod2; Liu, Zhi-Jie1; Stevens, Raymond C.1
2016-05
Source PublicationPROTEIN & CELL
ISSN1674-800X
Volume7Issue:5Pages:325-337
DOI10.1007/s13238-016-0263-8
AbstractG protein-coupled receptors (GPCRs) are involved in all humanphysiological systems where they are responsible for transducing extracellular signals into cells. GPCRs signal in response to a diverse array of stimuli including light, hormones, and lipids, where these signals affect downstream cascades to impact both health and disease states. Yet, despite their importance as therapeutic targets, detailed molecular structures of only 30 GPCRs have been determined to date. A key challenge to their structure determination is adequate protein expression. Here we report the quantification of protein expression in an insect cell expression system for all 826 human GPCRs using two different fusion constructs. Expression characteristics are analyzed in aggregate and among each of the five distinct subfamilies. These data can be used to identify trends related to GPCR expression between different fusion constructs and between different GPCR families, and to prioritize lead candidates for future structure determination feasibility.
KeywordG protein-coupled receptors insect protein expression surface expression analysis fusion construct
Indexed BySCI
Language英语
Funding ProjectNational Basic Research Program (973 Program)[2014CB910400] ; National Basic Research Program (973 Program)[2015CB910104]
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000379180100003
PublisherHIGHER EDUCATION PRESS
WOS KeywordCRYSTAL-STRUCTURE ; GPCR ; GLYCOSYLATION
Original Document TypeArticle
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1061
CollectioniHuman研究所_特聘教授组_Kurt Wuthrich组
iHuman研究所
iHuman研究所_特聘教授组_Raymond Stevens组
免疫化学研究所_公共科研平台_蛋白表达与制备平台
iHuman研究所_公共科研平台_Assay及筛选平台
iHuman研究所_公共科研平台_动物细胞培养和蛋白纯化平台
iHuman研究所_公共科研平台_昆虫细胞培养平台
iHuman研究所_公共科研平台_克隆平台
iHuman研究所_PI研究组_刘志杰组
iHuman研究所_PI研究组_徐菲组
iHuman研究所_PI研究组_陶厚朝组
生命科学与技术学院_硕士生
Corresponding AuthorStevens, Raymond C.
Affiliation1.ShanghaiTech Univ, IHuman Inst, Shanghai 201210, Peoples R China
2.Univ Southern Calif, Dept Biol Sci, Bridge Inst, Los Angeles, CA 90089 USA
First Author AffilicationiHuman Institute
Corresponding Author AffilicationiHuman Institute
First Signature AffilicationiHuman Institute
Recommended Citation
GB/T 7714
Lv, Xuechen,Liu, Junlin,Shi, Qiaoyun,et al. In vitro expression and analysis of the 826 human G protein-coupled receptors[J]. PROTEIN & CELL,2016,7(5):325-337.
APA Lv, Xuechen.,Liu, Junlin.,Shi, Qiaoyun.,Tan, Qiwen.,Wu, Dong.,...&Stevens, Raymond C..(2016).In vitro expression and analysis of the 826 human G protein-coupled receptors.PROTEIN & CELL,7(5),325-337.
MLA Lv, Xuechen,et al."In vitro expression and analysis of the 826 human G protein-coupled receptors".PROTEIN & CELL 7.5(2016):325-337.
Files in This Item: Download All
File Name/Size DocType Version Access License
10.1007@s13238-016-0(783KB)期刊论文出版稿开放获取CC BYView Download
Related Services
Usage statistics
Scholar Google
Similar articles in Scholar Google
[Lv, Xuechen]'s Articles
[Liu, Junlin]'s Articles
[Shi, Qiaoyun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Lv, Xuechen]'s Articles
[Liu, Junlin]'s Articles
[Shi, Qiaoyun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Lv, Xuechen]'s Articles
[Liu, Junlin]'s Articles
[Shi, Qiaoyun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 10.1007@s13238-016-0263-8.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.