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In vitro expression and analysis of the 826 human G protein-coupled receptors | |
2016-05 | |
Source Publication | PROTEIN & CELL
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ISSN | 1674-800X |
Volume | 7Issue:5Pages:325-337 |
Status | 已发表 |
DOI | 10.1007/s13238-016-0263-8 |
Abstract | G protein-coupled receptors (GPCRs) are involved in all humanphysiological systems where they are responsible for transducing extracellular signals into cells. GPCRs signal in response to a diverse array of stimuli including light, hormones, and lipids, where these signals affect downstream cascades to impact both health and disease states. Yet, despite their importance as therapeutic targets, detailed molecular structures of only 30 GPCRs have been determined to date. A key challenge to their structure determination is adequate protein expression. Here we report the quantification of protein expression in an insect cell expression system for all 826 human GPCRs using two different fusion constructs. Expression characteristics are analyzed in aggregate and among each of the five distinct subfamilies. These data can be used to identify trends related to GPCR expression between different fusion constructs and between different GPCR families, and to prioritize lead candidates for future structure determination feasibility. |
Keyword | G protein-coupled receptors insect protein expression surface expression analysis fusion construct |
Indexed By | SCI |
Language | 英语 |
Funding Project | National Basic Research Program (973 Program)[2014CB910400] ; National Basic Research Program (973 Program)[2015CB910104] |
WOS Research Area | Cell Biology |
WOS Subject | Cell Biology |
WOS ID | WOS:000379180100003 |
Publisher | HIGHER EDUCATION PRESS |
WOS Keyword | CRYSTAL-STRUCTURE ; GPCR ; GLYCOSYLATION |
Original Document Type | Article |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1061 |
Collection | iHuman研究所_特聘教授组_Kurt Wuthrich组 免疫化学研究所_特聘教授组_结构生物化学实验室 iHuman研究所 iHuman研究所_特聘教授组_Raymond Stevens组 免疫化学研究所_公共科研平台_蛋白表达与制备平台 iHuman研究所_公共科研平台_Assay及筛选平台 iHuman研究所_公共科研平台_动物细胞培养和蛋白纯化平台 iHuman研究所_公共科研平台_昆虫细胞培养平台 iHuman研究所_公共科研平台_克隆平台 iHuman研究所_PI研究组_刘志杰组 iHuman研究所_PI研究组_徐菲组 iHuman研究所_PI研究组_陶厚朝组 生命科学与技术学院_硕士生 |
Corresponding Author | Stevens, Raymond C. |
Affiliation | 1.ShanghaiTech Univ, IHuman Inst, Shanghai 201210, Peoples R China 2.Univ Southern Calif, Dept Biol Sci, Bridge Inst, Los Angeles, CA 90089 USA |
First Author Affilication | iHuman Institute |
Corresponding Author Affilication | iHuman Institute |
First Signature Affilication | iHuman Institute |
Recommended Citation GB/T 7714 | Lv, Xuechen,Liu, Junlin,Shi, Qiaoyun,et al. In vitro expression and analysis of the 826 human G protein-coupled receptors[J]. PROTEIN & CELL,2016,7(5):325-337. |
APA | Lv, Xuechen.,Liu, Junlin.,Shi, Qiaoyun.,Tan, Qiwen.,Wu, Dong.,...&Stevens, Raymond C..(2016).In vitro expression and analysis of the 826 human G protein-coupled receptors.PROTEIN & CELL,7(5),325-337. |
MLA | Lv, Xuechen,et al."In vitro expression and analysis of the 826 human G protein-coupled receptors".PROTEIN & CELL 7.5(2016):325-337. |
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