A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis
Dai, Peng1; Wang, Xin1; Gou, Lan-Tao1,2; Li, Zhi-Tong1; Wen, Ze1; Chen, Zong-Gui3; Hua, Min-Min1,4; Zhong, Ai1; Wang, Lingbo4,5; Su, Haiyang6; Wan, Huida7,8; Qian, Kun6; Liao, Lujian7,8; Li, Jinsong5,9; Tian, Bin10; Li, Dangsheng1; Fu, Xiang-Dong2; Shi, Hui-Juan4; Zhou, Yu3; Liu, Mo-Fang1,9
2019-12-12
Source PublicationCELL
ISSN0092-8674
Volume179Issue:7Pages:1566-+
Status已发表
DOI10.1016/j.cell.2019.11.022
AbstractSpermiogenesis is a highly orchestrated developmental process during which chromatin condensation decouples transcription from translation. Spermiogenic mRNAs are transcribed earlier and stored in a translationally inert state until needed for translation; however, it remains largely unclear how such repressed mRNAs become activated during spermiogenesis. We previously reported that the MIWI/piRNA machinery is responsible for mRNA elimination during late spermiogenesis in preparation for spermatozoa production. Here we unexpectedly discover that the same machinery is also responsible for activating translation of a subset of spermiogenic mRNAs to coordinate with morphological transformation into spermatozoa. Such action requires specific base-pairing interactions of piRNAs with target mRNAs in their 30 UTRs, which activates translation through coupling with cis-acting AU-rich elements to nucleate the formation of a MIWI/piRNA/eIF3f/HuR super-complex in a developmental stage-specific manner. These findings reveal a critical role of the piRNA system in translation activation, which we show is functionally required for spermatid development.
Indexed BySCI
Language英语
Funding ProjectNIH[GM052872] ; NIH[HG004659]
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology
WOS IDWOS:000502546200014
PublisherCELL PRESS
EISSN1097-4172
WOS KeywordRNA-BINDING-PROTEIN ; PIWI-INTERACTING RNA ; CELL SELF-RENEWAL ; MESSENGER-RNA ; MALE-INFERTILITY ; MIWI ; SEQUENCE ; UBIQUITINATION ; BIOGENESIS ; CLEAVAGE
Original Document TypeArticle
Citation statistics
Cited Times [WOS]:0   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://kms.shanghaitech.edu.cn/handle/2MSLDSTB/104997
Collection生命科学与技术学院_特聘教授组_李劲松组
生命科学与技术学院_特聘教授组_刘默芳组
Corresponding AuthorShi, Hui-Juan; Zhou, Yu; Liu, Mo-Fang
Affiliation1.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol,Ctr Excellence, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai 200031, Peoples R China
2.Univ Calif San Diego, Dept Cellular & Mol Med, La Jolla, CA 92093 USA
3.Wuhan Univ, Coll Life Sci, Inst Adv Studies, Wuhan 430072, Hubei, Peoples R China
4.Fudan Univ, Pharm Sch, Shanghai Inst Planned Parenthood Res, NHC Key Lab Reprod Regulat, Shanghai 200032, Peoples R China
5.Chinese Acad Sci, Univ Chinese Acad Sci, State Key Lab Cell Biol, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China
6.Shanghai Jiao Tong Univ, Sch Biomed Engn, Med X Res Inst, Shanghai 200030, Peoples R China
7.East China Normal Univ, Sch Life Sci, Shanghai Key Lab Regulatory Biol, Shanghai 200241, Peoples R China
8.East China Normal Univ, Sch Life Sci, Shanghai Key Lab Brain Funct Genom, Shanghai 200241, Peoples R China
9.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
10.Rutgers New Jersey Med Sch, Dept Microbiol Biochem & Mol Genet, Newark, NJ 07103 USA
Corresponding Author AffilicationSchool of Life Science and Technology
Recommended Citation
GB/T 7714
Dai, Peng,Wang, Xin,Gou, Lan-Tao,et al. A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis[J]. CELL,2019,179(7):1566-+.
APA Dai, Peng.,Wang, Xin.,Gou, Lan-Tao.,Li, Zhi-Tong.,Wen, Ze.,...&Liu, Mo-Fang.(2019).A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis.CELL,179(7),1566-+.
MLA Dai, Peng,et al."A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis".CELL 179.7(2019):1566-+.
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