A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis

doi:10.1016/j.cell.2019.11.022

	A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis
	Dai, Peng 1; Wang, Xin 1; Gou, Lan-Tao 1,2; Li, Zhi-Tong 1; Wen, Ze 1; Chen, Zong-Gui 3; Hua, Min-Min 1,4; Zhong, Ai 1; Wang, Lingbo 4,5; Su, Haiyang 6; Wan, Huida 7,8; Qian, Kun 6; Liao, Lujian 7,8; Li, Jinsong5,9 ; Tian, Bin 10; Li, Dangsheng 1; Fu, Xiang-Dong 2; Shi, Hui-Juan 4; Zhou, Yu 3; Liu, Mo-Fang1,9
	2019-12-12
发表期刊	CELL
ISSN	0092-8674
EISSN	1097-4172
卷号	179 期号:7 页码:1566-+
发表状态	已发表
DOI	10.1016/j.cell.2019.11.022
摘要	Spermiogenesis is a highly orchestrated developmental process during which chromatin condensation decouples transcription from translation. Spermiogenic mRNAs are transcribed earlier and stored in a translationally inert state until needed for translation; however, it remains largely unclear how such repressed mRNAs become activated during spermiogenesis. We previously reported that the MIWI/piRNA machinery is responsible for mRNA elimination during late spermiogenesis in preparation for spermatozoa production. Here we unexpectedly discover that the same machinery is also responsible for activating translation of a subset of spermiogenic mRNAs to coordinate with morphological transformation into spermatozoa. Such action requires specific base-pairing interactions of piRNAs with target mRNAs in their 30 UTRs, which activates translation through coupling with cis-acting AU-rich elements to nucleate the formation of a MIWI/piRNA/eIF3f/HuR super-complex in a developmental stage-specific manner. These findings reveal a critical role of the piRNA system in translation activation, which we show is functionally required for spermatid development.
收录类别	SCI ; SCIE
语种	英语
资助项目	NIH[GM052872] ; NIH[HG004659]
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000502546200014
出版者	CELL PRESS
WOS关键词	RNA-BINDING-PROTEIN ; PIWI-INTERACTING RNA ; CELL SELF-RENEWAL ; MESSENGER-RNA ; MALE-INFERTILITY ; MIWI ; SEQUENCE ; UBIQUITINATION ; BIOGENESIS ; CLEAVAGE
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/104997
专题	生命科学与技术学院_特聘教授组_李劲松组生命科学与技术学院_特聘教授组_刘默芳组生物医学工程学院
通讯作者	Shi, Hui-Juan; Zhou, Yu; Liu, Mo-Fang
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol,Ctr Excellence, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai 200031, Peoples R China 2.Univ Calif San Diego, Dept Cellular & Mol Med, La Jolla, CA 92093 USA 3.Wuhan Univ, Coll Life Sci, Inst Adv Studies, Wuhan 430072, Hubei, Peoples R China 4.Fudan Univ, Pharm Sch, Shanghai Inst Planned Parenthood Res, NHC Key Lab Reprod Regulat, Shanghai 200032, Peoples R China 5.Chinese Acad Sci, Univ Chinese Acad Sci, State Key Lab Cell Biol, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China 6.Shanghai Jiao Tong Univ, Sch Biomed Engn, Med X Res Inst, Shanghai 200030, Peoples R China 7.East China Normal Univ, Sch Life Sci, Shanghai Key Lab Regulatory Biol, Shanghai 200241, Peoples R China 8.East China Normal Univ, Sch Life Sci, Shanghai Key Lab Brain Funct Genom, Shanghai 200241, Peoples R China 9.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 10.Rutgers New Jersey Med Sch, Dept Microbiol Biochem & Mol Genet, Newark, NJ 07103 USA
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Dai, Peng,Wang, Xin,Gou, Lan-Tao,et al. A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis[J]. CELL,2019,179(7):1566-+.
APA	Dai, Peng.,Wang, Xin.,Gou, Lan-Tao.,Li, Zhi-Tong.,Wen, Ze.,...&Liu, Mo-Fang.(2019).A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis.CELL,179(7),1566-+.
MLA	Dai, Peng,et al."A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis".CELL 179.7(2019):1566-+.