SOX21 Ensures Rostral Forebrain Identity by Suppression of WNT8B during Neural Regionalization of Human Embryonic Stem Cells

doi:10.1016/j.stemcr.2019.10.013

	SOX21 Ensures Rostral Forebrain Identity by Suppression of WNT8B during Neural Regionalization of Human Embryonic Stem Cells
	Fang, Zhuoqing 1; Liu, Xinyuan 1; Wen, Jing 1; Tang, Fan 2; Zhou, Yang 1,5,6; Jing, Naihe3,4 ; Jin, Ying1,2,4
	2019-12-10
发表期刊	STEM CELL REPORTS (IF:5.9[JCR-2023],5.9[5-Year])
ISSN	2213-6711
卷号	13 期号:6 页码:1038-1052
发表状态	已发表
DOI	10.1016/j.stemcr.2019.10.013
摘要	The generation of brain region-specific progenitors from human embryonic stem cells (hESCs) is critical for their application. However, transcriptional regulation of neural regionalization in humans is poorly understood. Here, we applied a rostrocaudal patterning system from hESCs to dissect global transcriptional networks controlling early neural regionalization. We found that SOX21 is required for rostral forebrain fate specification. SOX21 knockout led to activation of Wnt signaling, resulting in caudalization of regional identity of rostral forebrain neural progenitor cells. Moreover, we identified WNT8B as a SOX21 direct target. Deletion of WNT8B or inhibition of Wnt signaling in SOX21 knockout neural progenitor cells restored rostral forebrain identity. Furthermore, SOX21 interacted with beta-catenin, interfering with the binding of TCF4/beta-catenin complex to the WNT8B enhancer. Collectively, these results unveil the unknown role of SOX21 and shed light on how a transcriptional factor modulates early neural regionalization through crosstalk with a key component of Wnt signaling.
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收录类别	SCI ; SCIE
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDB19020100]
WOS研究方向	Cell Biology
WOS类目	Cell & Tissue Engineering ; Cell Biology
WOS记录号	WOS:000502098700008
出版者	CELL PRESS
WOS关键词	HUMAN ES ; GENE-EXPRESSION ; SPECIFICATION ; REPRESSION ; NEURONS ; ANTEROPOSTERIOR ; DIFFERENTIATION ; PROGRESSION ; DISRUPTION ; PROTEINS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/104971
专题	生命科学与技术学院_特聘教授组_景乃禾组生命科学与技术学院_特聘教授组_金颖组
通讯作者	Jin, Ying
作者单位	1.Univ Chinese Acad Sci, CAS Key Lab Tissue Microenvironm & Tumor, CAS Ctr Excellence Mol Cell Sci,Chinese Acad Sci, Shanghai Inst Nutr & Hlth,Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China 2.Shanghai Jiao Tong Univ, Dept Histoembryol Genet & Dev Biol, Shanghai Key Lab Reprod Med, Basic Clin Res Ctr,Renji Hosp,Sch Med, 225 South Chongqing Rd, Shanghai 200025, Peoples R China 3.Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, State Key Lab Cell Biol,Chinese Acad Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China 5.Univ Alabama Birmingham, Sch Med, Dept Biomed Engn, Birmingham, AL 35294 USA 6.Univ Alabama Birmingham, Sch Engn, Birmingham, AL 35294 USA
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Fang, Zhuoqing,Liu, Xinyuan,Wen, Jing,et al. SOX21 Ensures Rostral Forebrain Identity by Suppression of WNT8B during Neural Regionalization of Human Embryonic Stem Cells[J]. STEM CELL REPORTS,2019,13(6):1038-1052.
APA	Fang, Zhuoqing.,Liu, Xinyuan.,Wen, Jing.,Tang, Fan.,Zhou, Yang.,...&Jin, Ying.(2019).SOX21 Ensures Rostral Forebrain Identity by Suppression of WNT8B during Neural Regionalization of Human Embryonic Stem Cells.STEM CELL REPORTS,13(6),1038-1052.
MLA	Fang, Zhuoqing,et al."SOX21 Ensures Rostral Forebrain Identity by Suppression of WNT8B during Neural Regionalization of Human Embryonic Stem Cells".STEM CELL REPORTS 13.6(2019):1038-1052.