Trio deep-sequencing does not reveal unexpected off-target and on-target mutations in Cas9-edited rhesus monkeys
Luo, Xin1,2,3; He, Yaoxi1,2,3; Zhang, Chao4; He, Xiechao1,3; Yan, Lanzhen3,5; Li, Min1,2; Hu, Ting1,2; Hu, Yan1,2; Jiang, Jin1,2; Meng, Xiaoyu1,2; Ji, Weizhi6; Zhao, Xudong3,5; Zheng, Ping1,7; Xu, Shuhua4,7,8,9; Su, Bing1,3,7
2019-12-04
Source PublicationNATURE COMMUNICATIONS
ISSN2041-1723
Volume10
Status已发表
DOI10.1038/s41467-019-13481-y
AbstractCRISPR-Cas9 is a widely-used genome editing tool, but its off-target effect and on-target complex mutations remain a concern, especially in view of future clinical applications. Non-human primates (NHPs) share close genetic and physiological similarities with humans, making them an ideal preclinical model for developing Cas9-based therapies. However, to our knowledge no comprehensive in vivo off-target and on-target assessment has been conducted in NHPs. Here, we perform whole genome trio sequencing of Cas9-treated rhesus monkeys. We only find a small number of de novo mutations that can be explained by expected spontaneous mutations, and no unexpected off-target mutations (OTMs) were detected. Furthermore, the long-read sequencing data does not detect large structural variants in the target region.
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Indexed BySCI
Language英语
Funding ProjectShanghai Municipal Science and Technology Major Project[2017SHZDZX01]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000500505100001
PublisherNATURE PUBLISHING GROUP
WOS KeywordREAD ALIGNMENT ; PAIRED-END ; CRISPR-CAS9 ; INSERTIONS ; GENERATION ; FRAMEWORK ; MODEL
Original Document TypeArticle
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Cited Times [WOS]:0   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://kms.shanghaitech.edu.cn/handle/2MSLDSTB/104510
Collection生命科学与技术学院_特聘教授组_徐书华组
Corresponding AuthorXu, Shuhua; Su, Bing
Affiliation1.Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Kunming Coll Life Sci, Beijing 100101, Peoples R China
3.Chinese Acad Sci, Kunming Primate Res Ctr, Kunming 650223, Yunnan, Peoples R China
4.Chinese Acad Sci, CAS MPG Partner Inst Computat Biol PICB, Shanghai Inst Biol Sci, Key Lab Computat Biol,Max Planck Independent Res, Shanghai 200031, Peoples R China
5.Chinese Acad Sci, Key Lab Anim Models & Human Dis Mech, Kunming Inst Zool, Kunming 650203, Yunnan, Peoples R China
6.Kunming Univ Sci & Technol, Inst Primate Translat Med, Yunnan Key Lab Primate Biomed Res, Kunming 650500, Yunnan, Peoples R China
7.Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming 650223, Yunnan, Peoples R China
8.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
9.Collaborat Innovat Ctr Genet & Dev, Shanghai 200438, Peoples R China
Corresponding Author AffilicationSchool of Life Science and Technology
Recommended Citation
GB/T 7714
Luo, Xin,He, Yaoxi,Zhang, Chao,et al. Trio deep-sequencing does not reveal unexpected off-target and on-target mutations in Cas9-edited rhesus monkeys[J]. NATURE COMMUNICATIONS,2019,10.
APA Luo, Xin.,He, Yaoxi.,Zhang, Chao.,He, Xiechao.,Yan, Lanzhen.,...&Su, Bing.(2019).Trio deep-sequencing does not reveal unexpected off-target and on-target mutations in Cas9-edited rhesus monkeys.NATURE COMMUNICATIONS,10.
MLA Luo, Xin,et al."Trio deep-sequencing does not reveal unexpected off-target and on-target mutations in Cas9-edited rhesus monkeys".NATURE COMMUNICATIONS 10(2019).
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