Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus
Yao, Run-Wen1; Xu, Guang1; Wang, Ying2; Shan, Lin1; Luan, Peng-Fei1; Wang, Yang1; Wu, Man1; Yang, Liang-Zhong1; Xing, Yu-Hang1,4,5; Yang, Li2,3; Chen, Ling-Ling1,3
2019-12-05
Source PublicationMOLECULAR CELL
ISSN1097-2765
Volume76Issue:5Pages:767-+
Status已发表
DOI10.1016/j.molcel.2019.08.014
AbstractFibrillar centers (FCs) and dense fibrillar components (DFCs) are essential morphologically distinct sub-regions of mammalian cell nucleoli for rDNA transcription and pre-rRNA processing. Here, we report that a human nucleolus consists of several dozen FC/DFC units, each containing 2-3 transcriptionally active rDNAs at the FC/DFC border. Pre-rRNA processing factors, such as fibrillarin (FBL), form 18-24 clusters that further assemble into the DFC surrounding the FC. Mechanistically, the 5' end of nascent 47S pre-rRNA binds co-transcriptionally to the RNA-binding domain of FBL. FBL diffuses to the DFC, where local self-association via its glycine- and arginine-rich (GAR) domain forms phase-separated clusters to immobilize FBL-interacting pre-rRNA, thus promoting directional traffic of nascent pre-rRNA while facilitating pre-rRNA processing and DFC formation. These results unveil FC/DFC ultrastructures in nucleoli and suggest a conceptual framework for considering nascent RNA sorting using multivalent interactions of their binding proteins.
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Indexed BySCI
Language英语
Funding ProjectHHMI[55008728]
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology
WOS IDWOS:000500937100009
PublisherCELL PRESS
EISSN1097-4164
WOS KeywordPOLYMERASE-I TRANSCRIPTION ; QUANTITATIVE-ANALYSIS ; ORGANIZER REGIONS ; SSU PROCESSOME ; BOX C/D ; BINDING ; HETEROCHROMATIN ; PROTEINS ; GENES ; ACTIVATION
Original Document TypeArticle
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://kms.shanghaitech.edu.cn/handle/2MSLDSTB/104497
Collection生命科学与技术学院_特聘教授组_杨力组
生命科学与技术学院_特聘教授组_陈玲玲组
Corresponding AuthorChen, Ling-Ling
Affiliation1.Chinese Acad Sci, Univ Chinese Acad Sci, State Key Lab Mol Biol,Shanghai Key Lab Mol Andro, CAS Ctr Excellence Mol Cell Sci,Shanghai Inst Bio, 320 Yueyang Rd, Shanghai 200031, Peoples R China
2.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Nutr & Hlth,Shanghai Inst Biol Sci, CAS Key Lab Computat Biol,CAS MPG Partner Inst Co, 320 Yueyang Rd, Shanghai 200031, Peoples R China
3.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China
4.Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA
5.Harvard Med Sch, Boston, MA 02114 USA
Corresponding Author AffilicationSchool of Life Science and Technology
Recommended Citation
GB/T 7714
Yao, Run-Wen,Xu, Guang,Wang, Ying,et al. Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus[J]. MOLECULAR CELL,2019,76(5):767-+.
APA Yao, Run-Wen.,Xu, Guang.,Wang, Ying.,Shan, Lin.,Luan, Peng-Fei.,...&Chen, Ling-Ling.(2019).Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus.MOLECULAR CELL,76(5),767-+.
MLA Yao, Run-Wen,et al."Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus".MOLECULAR CELL 76.5(2019):767-+.
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