Strategies for Developing CRISPR-Based Gene Editing Methods in Bacteria
Wu, Zhaowei; Wang, Yujue; Zhang, Yifei; Chen, Weizhong; Wang, Yu; Ji, Quanjiang
2019-11-19
Source PublicationSMALL METHODS
ISSN2366-9608
Status已发表
DOI10.1002/smtd.201900560
AbstractGene editing is a fundamental technique for the identification of the linkages from genetic determinants to significant biological phenotypes, and the engineering of industrial strains to produce fine chemicals. Recently, the primitive bacterial immunity systems, clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems, have been engineered as programmable nucleases to deliver double-strand breaks (DSBs) at user-defined loci. The DSB is repaired via either homology-direct repair or the non-homologous end joining pathway, generating a mutant in various organisms, and leading a revolution in the field of gene editing. This paper reviews the structural and molecular details of CRISPR-Cas systems, describing their applications and challenges of genome targeting in bacterial cells. Moreover, the DSB repair pathways in bacteria are summarized and a guideline for selecting repair machines and maximizing the recombination efficiency is presented. In addition, the plasmid curing approaches in bacteria are outlined for iterative gene editing or downstream biological applications. Furthermore, CRISPR-based transcriptional regulation, base editing, and transposition are also introduced. Finally, this paper prospects the future directions for improving CRISPR-based gene editing methods in bacteria.
Keywordbacteria base editing CRISPR-Cas gene editing recombination
Indexed BySCI
Language英语
Funding ProjectChina Postdoctoral Science Foundation[2019M651627]
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science
WOS SubjectChemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS IDWOS:000497110400001
PublisherWILEY-V C H VERLAG GMBH
WOS KeywordENGINEERING ESCHERICHIA-COLI ; SEQUENCE-SPECIFIC CONTROL ; DOUBLE-STRAND BREAKS ; R-LOOP COMPLEX ; CRYSTAL-STRUCTURE ; STAPHYLOCOCCUS-AUREUS ; STRUCTURAL BASIS ; RECBCD ENZYME ; PHAGE-LAMBDA ; MISMATCH REPAIR
Original Document TypeReview ; Early Access
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://kms.shanghaitech.edu.cn/handle/2MSLDSTB/102257
Collection物质科学与技术学院_PI研究组_季泉江组
物质科学与技术学院_硕士生
Corresponding AuthorJi, Quanjiang
AffiliationShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China
First Author AffilicationSchool of Physical Science and Technology
Corresponding Author AffilicationSchool of Physical Science and Technology
First Signature AffilicationSchool of Physical Science and Technology
Recommended Citation
GB/T 7714
Wu, Zhaowei,Wang, Yujue,Zhang, Yifei,et al. Strategies for Developing CRISPR-Based Gene Editing Methods in Bacteria[J]. SMALL METHODS,2019.
APA Wu, Zhaowei,Wang, Yujue,Zhang, Yifei,Chen, Weizhong,Wang, Yu,&Ji, Quanjiang.(2019).Strategies for Developing CRISPR-Based Gene Editing Methods in Bacteria.SMALL METHODS.
MLA Wu, Zhaowei,et al."Strategies for Developing CRISPR-Based Gene Editing Methods in Bacteria".SMALL METHODS (2019).
Files in This Item:
File Name/Size DocType Version Access License
10.1002@smtd.2019005(3790KB)期刊论文未知限制开放UnknownApplication Full Text
Related Services
Usage statistics
Scholar Google
Similar articles in Scholar Google
[Wu, Zhaowei]'s Articles
[Wang, Yujue]'s Articles
[Zhang, Yifei]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wu, Zhaowei]'s Articles
[Wang, Yujue]'s Articles
[Zhang, Yifei]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wu, Zhaowei]'s Articles
[Wang, Yujue]'s Articles
[Zhang, Yifei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.