Intra-Seminiferous Tubular Injection of Vascular Endothelial Growth Factor C Sustained-Release Ultrafine Particles: A Novel Method for Improving the Regeneration of Spermatogenesis After Chemotherapy
Zhao, Liangyu1; Yao, Chencheng1; Zhu, Zijue1; Liu, Nachuan1; Zhai, Jing1; Wang, Yizhou1; Yang, Chao1; Tian, Ruhui1; Li, Peng1; Chen, Huixing1; Wan, Zhong1; Zhi, Erlei1; Huang, Yuhua1; Zhou, Zhi2; Li, Zheng1
2019-12
Source PublicationJOURNAL OF BIOMEDICAL NANOTECHNOLOGY
ISSN1550-7033
Volume15Issue:12Pages:2376-2392
Status已发表
DOI10.1166/jbn.2019.2857
AbstractBusulfan and other chemotherapeutic drugs used in the treatment of cancer may result in temporary or even permanent damage to spermatogenesis. During spermatogenesis, the rapidly dividing spermatogonia are highly susceptible to chemotherapy. Consequently, there is significant interest in developing an approach that could provide stimulation and regenerate spermatogenesis after chemotherapy. In a previous study, we suggested the potential application for vascular endothelial growth factor C (VEGFC) because of its key role in stimulating the proliferation of spermatogonia. However, methods to facilitate the recovery of spermatogenesis in such patients using VEGFC, or other regulatory factors, are sorely lacking because of the rapid degradation of these proteins and restrictions created by the blood-testis- barrier. To this end, we loaded VEGFC into polyanion dextran sulfate incorporated in a polycation chitosan shell to produce VEGFC sustained-release ultrafine particles (UFPs, CS-DS-VEGFC). We tested such particles in an azoospermic mouse model, created using busulfan. For each mouse, CS-DS-VEGFC was injected into the seminiferous tubules of one testis, while unloaded UFPs (CS-DS), or the VEGFC protein alone, was injected into the opposite testis as a control. All mice were sacrificed and evaluated 5 weeks later. Spermatogenesis in the tubules that were injected with CS-DS-VEGFC was clearly better than those injected with controls, and contained more spermatogonia and spermatocytes, along with Ki67 and PCNA positive- cells per tubule. In addition, the phosphorylation levels of AKT and MAPK in these tubules were also higher than in controls, indicating that CS-DS-VEGFC could induce the sustained activation of these pathways. In conclusion, CS-DS-VEGFC, combined with the efferent tubule injection technique, is a feasible approach with which to improve the regeneration of spermatogenesis in busulfan-induced azoospermic mice.
KeywordVEGFC Ultrafine Particles Chemotherapy Spermatogenesis Regeneration Efferent Ductules Injection
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[81671512] ; National Natural Science Foundation of China[81701524] ; National Natural Science Foundation of China[81701428]
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Biomaterials
WOS IDWOS:000498023900007
PublisherAMER SCIENTIFIC PUBLISHERS
EISSN1550-7041
WOS KeywordSPERMATOGONIAL STEM-CELLS ; PROLIFERATION ; DIFFERENTIATION ; NANOPARTICLES ; DRUGS ; KIT ; TRANSPLANTATION ; ACTIVATION ; EXPRESSION ; APOPTOSIS
Original Document TypeArticle
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Cited Times [WOS]:0   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://kms.shanghaitech.edu.cn/handle/2MSLDSTB/102252
Collection生命科学与技术学院_PI研究组_周智组
Corresponding AuthorZhou, Zhi; Li, Zheng
Affiliation1.Shanghai Jiao Tong Univ, Sch Med, Urol Med Ctr,Shanghai Gen Hosp,Shanghai Key Lab R, Dept Androl,Ctr Mens Hlth,Inst Urol, Shanghai 200080, Peoples R China
2.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 200120, Peoples R China
Corresponding Author AffilicationSchool of Life Science and Technology
Recommended Citation
GB/T 7714
Zhao, Liangyu,Yao, Chencheng,Zhu, Zijue,et al. Intra-Seminiferous Tubular Injection of Vascular Endothelial Growth Factor C Sustained-Release Ultrafine Particles: A Novel Method for Improving the Regeneration of Spermatogenesis After Chemotherapy[J]. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2019,15(12):2376-2392.
APA Zhao, Liangyu.,Yao, Chencheng.,Zhu, Zijue.,Liu, Nachuan.,Zhai, Jing.,...&Li, Zheng.(2019).Intra-Seminiferous Tubular Injection of Vascular Endothelial Growth Factor C Sustained-Release Ultrafine Particles: A Novel Method for Improving the Regeneration of Spermatogenesis After Chemotherapy.JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,15(12),2376-2392.
MLA Zhao, Liangyu,et al."Intra-Seminiferous Tubular Injection of Vascular Endothelial Growth Factor C Sustained-Release Ultrafine Particles: A Novel Method for Improving the Regeneration of Spermatogenesis After Chemotherapy".JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 15.12(2019):2376-2392.
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