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Intra-Seminiferous Tubular Injection of Vascular Endothelial Growth Factor C Sustained-Release Ultrafine Particles: A Novel Method for Improving the Regeneration of Spermatogenesis After Chemotherapy | |
Zhao, Liangyu1; Yao, Chencheng1; Zhu, Zijue1; Liu, Nachuan1; Zhai, Jing1; Wang, Yizhou1; Yang, Chao1; Tian, Ruhui1; Li, Peng1; Chen, Huixing1 | |
2019-12 | |
发表期刊 | JOURNAL OF BIOMEDICAL NANOTECHNOLOGY |
ISSN | 1550-7033 |
EISSN | 1550-7041 |
卷号 | 15期号:12页码:2376-2392 |
发表状态 | 已发表 |
DOI | 10.1166/jbn.2019.2857 |
摘要 | Busulfan and other chemotherapeutic drugs used in the treatment of cancer may result in temporary or even permanent damage to spermatogenesis. During spermatogenesis, the rapidly dividing spermatogonia are highly susceptible to chemotherapy. Consequently, there is significant interest in developing an approach that could provide stimulation and regenerate spermatogenesis after chemotherapy. In a previous study, we suggested the potential application for vascular endothelial growth factor C (VEGFC) because of its key role in stimulating the proliferation of spermatogonia. However, methods to facilitate the recovery of spermatogenesis in such patients using VEGFC, or other regulatory factors, are sorely lacking because of the rapid degradation of these proteins and restrictions created by the blood-testis- barrier. To this end, we loaded VEGFC into polyanion dextran sulfate incorporated in a polycation chitosan shell to produce VEGFC sustained-release ultrafine particles (UFPs, CS-DS-VEGFC). We tested such particles in an azoospermic mouse model, created using busulfan. For each mouse, CS-DS-VEGFC was injected into the seminiferous tubules of one testis, while unloaded UFPs (CS-DS), or the VEGFC protein alone, was injected into the opposite testis as a control. All mice were sacrificed and evaluated 5 weeks later. Spermatogenesis in the tubules that were injected with CS-DS-VEGFC was clearly better than those injected with controls, and contained more spermatogonia and spermatocytes, along with Ki67 and PCNA positive- cells per tubule. In addition, the phosphorylation levels of AKT and MAPK in these tubules were also higher than in controls, indicating that CS-DS-VEGFC could induce the sustained activation of these pathways. In conclusion, CS-DS-VEGFC, combined with the efferent tubule injection technique, is a feasible approach with which to improve the regeneration of spermatogenesis in busulfan-induced azoospermic mice. |
关键词 | VEGFC Ultrafine Particles Chemotherapy Spermatogenesis Regeneration Efferent Ductules Injection |
收录类别 | SCI ; SCIE |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[81671512] ; National Natural Science Foundation of China[81701524] ; National Natural Science Foundation of China[81701428] |
WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
WOS类目 | Nanoscience & Nanotechnology ; Materials Science, Biomaterials |
WOS记录号 | WOS:000498023900007 |
出版者 | AMER SCIENTIFIC PUBLISHERS |
WOS关键词 | SPERMATOGONIAL STEM-CELLS ; PROLIFERATION ; DIFFERENTIATION ; NANOPARTICLES ; DRUGS ; KIT ; TRANSPLANTATION ; ACTIVATION ; EXPRESSION ; APOPTOSIS |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/102252 |
专题 | 生命科学与技术学院_PI研究组_周智组 |
通讯作者 | Zhou, Zhi; Li, Zheng |
作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Urol Med Ctr,Shanghai Gen Hosp,Shanghai Key Lab R, Dept Androl,Ctr Mens Hlth,Inst Urol, Shanghai 200080, Peoples R China 2.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 200120, Peoples R China |
通讯作者单位 | 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Zhao, Liangyu,Yao, Chencheng,Zhu, Zijue,et al. Intra-Seminiferous Tubular Injection of Vascular Endothelial Growth Factor C Sustained-Release Ultrafine Particles: A Novel Method for Improving the Regeneration of Spermatogenesis After Chemotherapy[J]. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2019,15(12):2376-2392. |
APA | Zhao, Liangyu.,Yao, Chencheng.,Zhu, Zijue.,Liu, Nachuan.,Zhai, Jing.,...&Li, Zheng.(2019).Intra-Seminiferous Tubular Injection of Vascular Endothelial Growth Factor C Sustained-Release Ultrafine Particles: A Novel Method for Improving the Regeneration of Spermatogenesis After Chemotherapy.JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,15(12),2376-2392. |
MLA | Zhao, Liangyu,et al."Intra-Seminiferous Tubular Injection of Vascular Endothelial Growth Factor C Sustained-Release Ultrafine Particles: A Novel Method for Improving the Regeneration of Spermatogenesis After Chemotherapy".JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 15.12(2019):2376-2392. |
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