Intra-Seminiferous Tubular Injection of Vascular Endothelial Growth Factor C Sustained-Release Ultrafine Particles: A Novel Method for Improving the Regeneration of Spermatogenesis After Chemotherapy
Zhao, Liangyu1; Yao, Chencheng1; Zhu, Zijue1; Liu, Nachuan1; Zhai, Jing1; Wang, Yizhou1; Yang, Chao1; Tian, Ruhui1; Li, Peng1; Chen, Huixing1
2019-12
发表期刊JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
ISSN1550-7033
EISSN1550-7041
卷号15期号:12页码:2376-2392
发表状态已发表
DOI10.1166/jbn.2019.2857
摘要Busulfan and other chemotherapeutic drugs used in the treatment of cancer may result in temporary or even permanent damage to spermatogenesis. During spermatogenesis, the rapidly dividing spermatogonia are highly susceptible to chemotherapy. Consequently, there is significant interest in developing an approach that could provide stimulation and regenerate spermatogenesis after chemotherapy. In a previous study, we suggested the potential application for vascular endothelial growth factor C (VEGFC) because of its key role in stimulating the proliferation of spermatogonia. However, methods to facilitate the recovery of spermatogenesis in such patients using VEGFC, or other regulatory factors, are sorely lacking because of the rapid degradation of these proteins and restrictions created by the blood-testis- barrier. To this end, we loaded VEGFC into polyanion dextran sulfate incorporated in a polycation chitosan shell to produce VEGFC sustained-release ultrafine particles (UFPs, CS-DS-VEGFC). We tested such particles in an azoospermic mouse model, created using busulfan. For each mouse, CS-DS-VEGFC was injected into the seminiferous tubules of one testis, while unloaded UFPs (CS-DS), or the VEGFC protein alone, was injected into the opposite testis as a control. All mice were sacrificed and evaluated 5 weeks later. Spermatogenesis in the tubules that were injected with CS-DS-VEGFC was clearly better than those injected with controls, and contained more spermatogonia and spermatocytes, along with Ki67 and PCNA positive- cells per tubule. In addition, the phosphorylation levels of AKT and MAPK in these tubules were also higher than in controls, indicating that CS-DS-VEGFC could induce the sustained activation of these pathways. In conclusion, CS-DS-VEGFC, combined with the efferent tubule injection technique, is a feasible approach with which to improve the regeneration of spermatogenesis in busulfan-induced azoospermic mice.
关键词VEGFC Ultrafine Particles Chemotherapy Spermatogenesis Regeneration Efferent Ductules Injection
收录类别SCI ; SCIE
语种英语
资助项目National Natural Science Foundation of China[81671512] ; National Natural Science Foundation of China[81701524] ; National Natural Science Foundation of China[81701428]
WOS研究方向Science & Technology - Other Topics ; Materials Science
WOS类目Nanoscience & Nanotechnology ; Materials Science, Biomaterials
WOS记录号WOS:000498023900007
出版者AMER SCIENTIFIC PUBLISHERS
WOS关键词SPERMATOGONIAL STEM-CELLS ; PROLIFERATION ; DIFFERENTIATION ; NANOPARTICLES ; DRUGS ; KIT ; TRANSPLANTATION ; ACTIVATION ; EXPRESSION ; APOPTOSIS
原始文献类型Article
引用统计
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/102252
专题生命科学与技术学院_PI研究组_周智组
通讯作者Zhou, Zhi; Li, Zheng
作者单位1.Shanghai Jiao Tong Univ, Sch Med, Urol Med Ctr,Shanghai Gen Hosp,Shanghai Key Lab R, Dept Androl,Ctr Mens Hlth,Inst Urol, Shanghai 200080, Peoples R China
2.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 200120, Peoples R China
通讯作者单位生命科学与技术学院
推荐引用方式
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Zhao, Liangyu,Yao, Chencheng,Zhu, Zijue,et al. Intra-Seminiferous Tubular Injection of Vascular Endothelial Growth Factor C Sustained-Release Ultrafine Particles: A Novel Method for Improving the Regeneration of Spermatogenesis After Chemotherapy[J]. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2019,15(12):2376-2392.
APA Zhao, Liangyu.,Yao, Chencheng.,Zhu, Zijue.,Liu, Nachuan.,Zhai, Jing.,...&Li, Zheng.(2019).Intra-Seminiferous Tubular Injection of Vascular Endothelial Growth Factor C Sustained-Release Ultrafine Particles: A Novel Method for Improving the Regeneration of Spermatogenesis After Chemotherapy.JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,15(12),2376-2392.
MLA Zhao, Liangyu,et al."Intra-Seminiferous Tubular Injection of Vascular Endothelial Growth Factor C Sustained-Release Ultrafine Particles: A Novel Method for Improving the Regeneration of Spermatogenesis After Chemotherapy".JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 15.12(2019):2376-2392.
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