Independent manipulation of histone H3 modifications in individual nucleosomes reveals the contributions of sister histones to transcription

doi:10.7554/eLife.30178

	Independent manipulation of histone H3 modifications in individual nucleosomes reveals the contributions of sister histones to transcription
	Zhou, Zhen 1; Liu, Yu-Ting 1; Ma, Li 2; Gong, Ting 1; Hu, Ya-Nan 2; Li, Hong-Tao 1; Cai, Chen1,3 ; Zhang, Ling-Li 1; Wei, Gang 2; Zhou, Jin-Qiu1,3
	2017-10-13
发表期刊	ELIFE
ISSN	2050-084X
卷号	6
发表状态	已发表
DOI	10.7554/eLife.30178
摘要	Histone tail modifications can greatly influence chromatin-associated processes. Asymmetrically modified nucleosomes exist in multiple cell types, but whether modifications on both sister histones contribute equally to chromatin dynamics remains elusive. Here, we devised a bivalent nucleosome system that allowed for the constitutive assembly of asymmetrically modified sister histone H3s in nucleosomes in Saccharomyces cerevisiae. The sister H3K36 methylations independently affected cryptic transcription in gene coding regions, whereas sister H3K79 methylation had cooperative effects on gene silencing near telomeres. H3K4 methylation on sister histones played an independent role in suppressing the recruitment of Gal4 activator to the GAL1 promoter and in inhibiting GAL1 transcription. Under starvation stress, sister H3K4 methylations acted cooperatively, independently or redundantly to regulate transcription. Thus, we provide a unique tool for comparing symmetrical and asymmetrical modifications of sister histone H3s in vivo.
收录类别	SCI
语种	英语
资助项目	Ministry of Science and Technology[MOST 2016YFA0500701]
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
WOS记录号	WOS:000414742000001
出版者	ELIFE SCIENCES PUBLICATIONS LTD
WOS关键词	ASYMMETRICALLY MODIFIED NUCLEOSOMES ; LARGE GENE LISTS ; SACCHAROMYCES-CEREVISIAE ; CHROMATIN-STRUCTURE ; CORE PARTICLE ; LYSINE 4 ; CRYSTAL-STRUCTURE ; CODING REGIONS ; METHYLATION ; YEAST
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/10058
专题	生命科学与技术学院_博士生生命科学与技术学院生命科学与技术学院_特聘教授组_周金秋组
通讯作者	Zhou, Jin-Qiu
作者单位	1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol,CAS Ctr Excelle, Innovat Ctr Cell Signaling Network,State Key Lab, Shanghai, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai, Peoples R China 3.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhou, Zhen,Liu, Yu-Ting,Ma, Li,et al. Independent manipulation of histone H3 modifications in individual nucleosomes reveals the contributions of sister histones to transcription[J]. ELIFE,2017,6.
APA	Zhou, Zhen.,Liu, Yu-Ting.,Ma, Li.,Gong, Ting.,Hu, Ya-Nan.,...&Zhou, Jin-Qiu.(2017).Independent manipulation of histone H3 modifications in individual nucleosomes reveals the contributions of sister histones to transcription.ELIFE,6.
MLA	Zhou, Zhen,et al."Independent manipulation of histone H3 modifications in individual nucleosomes reveals the contributions of sister histones to transcription".ELIFE 6(2017).