Functional and Structural Characterization of a Receptor-Like Kinase Involved in Germination and Cell Expansion in Arabidopsis

doi:10.3389/fpls.2017.01999

KMS > 免疫化学研究所 > 特聘教授组 > Roger D. Kornberg组

	Functional and Structural Characterization of a Receptor-Like Kinase Involved in Germination and Cell Expansion in Arabidopsis; Functional and Structural Characterization of a Receptor-Like Kinase Involved in Germination and Cell Expansion in Arabidopsis
	Wu, Zhen 1; Liang, Shan 2; Song, Wen 1,3,4; Lin, Guangzhong 1; Wang, Weiguang 1; Zhang, Heqiao5 ; Han, Zhifu 1; Chai, Jijie 1,3,4
	2017-11-22 ; 2017-11-22
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X ; 1664-462X
卷号	8
发表状态	已发表 ; 已发表
DOI	10.3389/fpls.2017.01999 ; 10.3389/fpls.2017.01999
摘要	Leucine-rich repeat receptor-like kinases (LRR-RLKs) are widespread in different plant species and play important roles in growth and development. Germination inhibition is vital for the completion of seed maturation and cell expansion is a fundamental cellular process driving plant growth. Here, we report genetic and structural characterizations of a functionally uncharacterized LRR-RLK, named GRACE (Germination Repression and Cell Expansion receptor-like kinase). Overexpression of GRACE in Arabidopsis exhibited delayed germination, enlarged cotyledons, rosette leaves and stubbier petioles. Conversely, these phenotypes were reversed in the T-DNA insertion knock-down mutant grace-1 plants. A crystal structure of the extracellular domain of GRACE (GRACE-LRR) determined at the resolution of 3.0 angstrom revealed that GRACE-LRR assumed a right-handed super-helical structure with an island domain (ID). Structural comparison showed that structure of the ID in GRACE-LRR is strikingly different from those observed in other LRR-RLKs. This structural observation implies that GRACE might perceive a new ligand for signaling. Collectively, our data support roles of GRACE in repressing seed germination and promoting cell expansion of Arabidopsis, presumably by perception of unknown ligand(s).; Leucine-rich repeat receptor-like kinases (LRR-RLKs) are widespread in different plant species and play important roles in growth and development. Germination inhibition is vital for the completion of seed maturation and cell expansion is a fundamental cellular process driving plant growth. Here, we report genetic and structural characterizations of a functionally uncharacterized LRR-RLK, named GRACE (Germination Repression and Cell Expansion receptor-like kinase). Overexpression of GRACE in Arabidopsis exhibited delayed germination, enlarged cotyledons, rosette leaves and stubbier petioles. Conversely, these phenotypes were reversed in the T-DNA insertion knock-down mutant grace-1 plants. A crystal structure of the extracellular domain of GRACE (GRACE-LRR) determined at the resolution of 3.0 angstrom revealed that GRACE-LRR assumed a right-handed super-helical structure with an island domain (ID). Structural comparison showed that structure of the ID in GRACE-LRR is strikingly different from those observed in other LRR-RLKs. This structural observation implies that GRACE might perceive a new ligand for signaling. Collectively, our data support roles of GRACE in repressing seed germination and promoting cell expansion of Arabidopsis, presumably by perception of unknown ligand(s).
关键词	GRACE GRACE LRR-RLKs ,cell expansion LRR-RLKs ,cell expansion seed germination seed germination Island domain Island domain
收录类别	SCI ; SCI
语种	英语 ; 英语
资助项目	Project of International Cooperation and Exchanges NNSFC[31600205] ; Project of International Cooperation and Exchanges NNSFC[31600205]
WOS研究方向	Plant Sciences ; Plant Sciences
WOS类目	Plant Sciences ; Plant Sciences
WOS记录号	WOS:000415916800002 ; WOS:000415916800002
出版者	FRONTIERS MEDIA SA ; FRONTIERS MEDIA SA
WOS关键词	LEUCINE-RICH REPEAT ; LEUCINE-RICH REPEAT ; MEMBRANE H+-ATPASE ; MEMBRANE H+-ATPASE ; TRANSCRIPTION FACTORS ; TRANSCRIPTION FACTORS ; ABSCISIC-ACID ; ABSCISIC-ACID ; CRYSTAL-STRUCTURE ; CRYSTAL-STRUCTURE ; EXTRACELLULAR DOMAIN ; EXTRACELLULAR DOMAIN ; NEGATIVE REGULATOR ; NEGATIVE REGULATOR ; STRESS TOLERANCE ; STRESS TOLERANCE ; PEPTIDE-HORMONE ; PEPTIDE-HORMONE ; PROTEIN-KINASE ; PROTEIN-KINASE
原始文献类型	Article ; Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/10054
专题	免疫化学研究所_特聘教授组_结构生物化学实验室
通讯作者	Chai, Jijie
作者单位	1.Tsinghua Univ, Tsinghua Peking Joint Ctr Life Sci, Innovat Ctr Struct Biol, Sch Life Sci, Beijing, Peoples R China 2.Beijing Technol & Business Univ, Sch Food & Chem Engn, Beijing, Peoples R China 3.Max Planck Inst Plant Breeding Res, Cologne, Germany 4.Univ Cologne, Inst Biochem, Cologne, Germany 5.Shanghai Tech Univ, Sch Life Sci & Thechol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Wu, Zhen,Liang, Shan,Song, Wen,et al. Functional and Structural Characterization of a Receptor-Like Kinase Involved in Germination and Cell Expansion in Arabidopsis, Functional and Structural Characterization of a Receptor-Like Kinase Involved in Germination and Cell Expansion in Arabidopsis[J]. FRONTIERS IN PLANT SCIENCE,2017, 2017,8, 8.
APA	Wu, Zhen.,Liang, Shan.,Song, Wen.,Lin, Guangzhong.,Wang, Weiguang.,...&Chai, Jijie.(2017).Functional and Structural Characterization of a Receptor-Like Kinase Involved in Germination and Cell Expansion in Arabidopsis.FRONTIERS IN PLANT SCIENCE,8.
MLA	Wu, Zhen,et al."Functional and Structural Characterization of a Receptor-Like Kinase Involved in Germination and Cell Expansion in Arabidopsis".FRONTIERS IN PLANT SCIENCE 8(2017).